The workflow of antibody discovery involves variations on immunization, isolation of B-cells from blood, plating of single viable cells, and sequencing of cells. The WOLF Cell Sorter enhances key steps in this workflow. First, by sorting B-cells from blood, the relevant cells can be collected for downstream characterization and plating. Once reactive B-cells are identified, gentle, sterile sorting and single-cell plating into 96- and 384-well plates for expansion, further characterization, and sequencing can be performed in the any lab. The WOLF provides easy-to-use cell enrichment and single-cell dispensing without the need for flow cytometry expertise. The robust, portable, sterile, and safe system can be used in any lab without the need for dedicated hoods, venting, or personnel. The rapidly-exchangeable cartridge and tubing set can easily set-up and taken down in minutes to eliminate sample carry over and time consuming cleaning protocols.