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    Click on the links below to explore the power of modern and simple cell sorting

    Department of Ophthalmology, UCSD

    Traditionally, Retinal Ganglion Cells (RGCs) have been difficult to sort due to their fragile nature, but the WOLF Cell Sorter has enabled the ability to sort sensitive cell types and prepare a higher quality sample than conventional practices.

    Sanford Consortium for Regenerative Medicine, UCSD

    Human iPS cells (hiPSC) can be modified utilizing CRISPR/Cas9 gene editing technology to generate in vitro models of human diseases, but there are critical shortcomings in the generation of CRISPR-modified clonal lines. See how microfluidic cell sorting can improve overall results compared to a droplet sorter.

    Gentle cell sorting with the WOLF improves cell line development, with customers, like Atum, normally getting 80% single cells per well and high outgrowth.

    Cloning bovine immunoglobulin VH and VL genes from single B cells (Poster accepted at Antibody Engineering & Therapeutics)

    Scripps Research, Department of Molecular Medicine and Applied Biomedical Science Institute

    Bovine immunoglobulins with ultralong CDR H3s have great potential for development as clinical treatments and research tools targeting a broad variety of antigens. However, unlike other species, techniques for studying bovine immunoglobulin genes at the single cell level have not been comprehensively developed. The Smider Lab has established a new method for amplification of bovine immunoglobulin VH and VL genes at the single cell level utilizing flow cytometry to sort individual B cells into wells and cDNA production and PCR amplification followed by nested PCR.

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